Part:BBa_K555009:Design

pYES2 - galactose-inducible expression vector for yeast

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 550
  Illegal XbaI site found at 595
  Illegal SpeI site found at 525
  Illegal SpeI site found at 5851
  Illegal PstI site found at 555
  Illegal PstI site found at 3632
  Illegal PstI site found at 4120
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 550
  Illegal NheI site found at 3839
  Illegal SpeI site found at 525
  Illegal SpeI site found at 5851
  Illegal PstI site found at 555
  Illegal PstI site found at 3632
  Illegal PstI site found at 4120
  Illegal NotI site found at 576
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 550
  Illegal BamHI site found at 519
  Illegal XhoI site found at 583
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 550
  Illegal XbaI site found at 595
  Illegal SpeI site found at 525
  Illegal SpeI site found at 5851
  Illegal PstI site found at 555
  Illegal PstI site found at 3632
  Illegal PstI site found at 4120
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 550
  Illegal XbaI site found at 595
  Illegal SpeI site found at 525
  Illegal SpeI site found at 5851
  Illegal PstI site found at 555
  Illegal PstI site found at 3632
  Illegal PstI site found at 4120
  Illegal NgoMIV site found at 5709
  Illegal AgeI site found at 71
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1996
  Illegal BsaI.rc site found at 2942
  Illegal SapI site found at 913
  Illegal SapI site found at 3094
  

Design Notes

n/a

Source

n/a